|Statement||papers by Charles E. Hamner, et al.|
|Contributions||Hamner, Charles E.|
|LC Classifications||QP273 .S64 1973|
|The Physical Object|
|Number of Pages||230|
|LC Control Number||72013501|
COVID Resources. Reliable information about the coronavirus (COVID) is available from the World Health Organization (current situation, international travel).Numerous and frequently-updated resource results are available from this ’s WebJunction has pulled together information and resources to assist library staff as they consider how to handle coronavirus. Introduction Sperm capacitation represents the culmination of a spermatozoon’s journey to functional maturity that begins within the testes (spermatogenesis) and continues as the cells are conveyed through the male (epididymal maturation) and female (capacitation) reproductive tracts. The sperm must undergo capacitation in the female’s reproductive tract over several hours, which increases its motility and destabilizes its membrane. By destabilizing the membrane, the sperm prepares for the acrosome reaction, the enzymatic penetration of the . sperm under a variety of different incubation conditions. His-torically, capacitation was originally deﬁned as the time interval of sperm incubation (either in vivo or in vitro) that is required to bring about this ﬁnal functional maturation of the sperm (Chang, ). This loose deﬁnition takes into.
Sperm movement through the Fallopian tube relies on a combination of forces: intrinsic sperm motility, tubular muscular contraction, and fluid flow. Tubal fluid production is maximal at the time of ovulation, and this fluid sustains the sperm before fertilization. 50 Tubal fluid may also facilitate both sperm capacitation and acrosomal reaction. Abstract. To achieve successful fertilization under normal circumstances in vivo, mammalian spermatozoa must first undergo capacitation and then the acrosome reaction, an exocytotic event that allows cells to penetrate the zona pellucida and fuse with the oocyte plasma complex events permit spermatozoa to achieve fertilizing ability at the right time in the right place. Capacitation is the penultimate step in the maturation of mammalian spermatozoa and is required to render them competent to fertilize an oocyte. This step is a biochemical event; the sperm move normally and look mature prior to capacitation. In vivo, capacitation occurs after ejaculation, when the spermatozoa leave the vagina and enter the superior female reproductive tract. The conclusion reached by both investigators was that sperm must reside within the oviduct for some time prior to ovulation to acquire fertilizing ability. The acquisition of fertilizing ability, termed capacitation by Austin (), is now recognized as an essential feature of mammalian fertilization.
Phospholipase C zeta undergoes dynamic changes in its pattern of localization in sperm during capacitation and the acrosome reaction. Fertil Steril 91 (): – Capacitation is the activation process that prepares the sperm for fertilizing the egg and begins once the sperm enter the upper female genital tract or uterus. The process prepares the sperm to undergo the acrosome reaction, which will allow it to penetrate the outer layer of the egg for fertilization. Most of the studies done on sperm capacitation are done in vitro. disadvantage of not completely mimicking conditions in vivo; since sperm suspensions have tendency to undergo SAR even when not in presence of egg. Suggesting that internal in vivo environment provides environment to hold cells in . The sperm are prepared by washing to remove seminal fluid because seminal fluid contains a peptide, FPP (or, fertilization promoting peptide), that—in high concentrations—prevents capacitation of the sperm. The sperm sample is also concentrated, to increase the sperm count per milliliter. Next, the eggs and sperm are mixed in a petri dish.